Fig. 1: Capsule serotype determines phage infectivity.
From: Capsules and their traits shape phage susceptibility and plasmid conjugation efficiency
A Overview of the genetic loci encoding the four different capsule serotypes included in this study and their chemical composition. Arrows represent the different genes, galF and ugd in blue corresponding to the regions involved in homologous recombination to generate the swaps. Conserved genes involved in assembly and export of the capsule (wza, wzb, wzc, wzi) and initiating glycosyl-transferase (wcaJ, wbaP) are labelled. The chemical composition of the capsule (monomers and their organisation), is displayed on the right of each locus (predicted by K-PAM129). B Matrix of phage infection. Infection assay for each of the three phages (panels), three swapped strains (y-axis), and different genotypes (x-axis). White tiles correspond to non-productive infection, i.e., no plaque could be identified. Coloured tiles correspond to the average PFU/mL normalized by the lysate titre for productive infections (Supplementary Fig. S2). Grey tiles correspond to non-productive infection with significant adsorption, while white tiles correspond to non-adsorptive pairs (Supplementary Fig. S3). Values are the mean of three independent replicates after log10-transformation. Strain-specific defence systems identified by DefenseFinder as of 02/202363 are displayed on the right. Source data are provided as a Source Data file 1 (Adsorption) and Source Data file 2 (Infection).
