Fig. 5: IL-1α contributes to the neutrophil accumulation in the IgE-CAI skin lesion of Ccr2−/− mice. | Nature Communications

Fig. 5: IL-1α contributes to the neutrophil accumulation in the IgE-CAI skin lesion of Ccr2−/− mice.

From: Single-cell transcriptomics identifies the differentiation trajectory from inflammatory monocytes to pro-resolving macrophages in a mouse skin allergy model

Fig. 5

WT and Ccr2−/− BALB/c mice were treated as in Fig. 1 to induce IgE-CAI. a Anakinra (human recombinant IL-1 antagonist) or control PBS was intraperitoneally administered to indicated mice on days 0, 1, 2, 3, and 4 post-challenge. The time course of ear swelling (Δ ear thickness) is shown (mean ± SEM, n = 3 biologically independent animals for each group). *p = 0.0371 (for day 1), *p = 0.0274 (for day 2), ****p = 1.89×10-5 (for day 3), ****p = 7.98 × 10−7 (for day 4), and ****p = 1.19 × 10−9 (for day 5) measured by two-way ANOVA with Tukey’s multiple comparison test. b Neutralizing antibody against IL-1α, IL-1β or its isotype-matched control antibody (HmIgG) was intraperitoneally administered to indicated mice on days 0, 1, 2, 3, and 4 post-challenge. Time course of ear swelling (Δ ear thickness) is shown (mean ± SEM, n = 3 biologically independent animals for each group). In the left panel, *p = 0.0219 (for day 1), **p = 0.0049 (for day 2), ****p = 3.74 × 10−7 (for day 3), ****p = 1.16 × 10−7 (for day 4), and ****p = 4.86 × 10−8 (for day 5) measured by two-way ANOVA with Tukey’s multiple comparison test. c The number of hematopoietic cells and neutrophils in the ear skin of indicated mice on day 5 is shown (mean ± SEM, n = 4 biologically independent animals for WT HmIgG and Ccr2−/− HmIgG groups; n = 3 biologically independent animals for TNP-OVA-treated Ccr2−/− anti-IL-1α group; n = 2 biologically independent animals for OVA-treated Ccr2−/− anti-IL-1α group). In the left panel, ****p = 1.51 × 10-8 (WT HmIgG vs. Ccr2−/− HmIgG), **p = 0.0032 (Ccr2−/− HmIgG vs. Ccr2−/− anti-IL-1α) measured by two-way ANOVA with Tukey’s multiple comparison test. In the right panel ****p = 2.46 × 10−8 (WT HmIgG vs. Ccr2−/− HmIgG), ***p = 0.001 (Ccr2−/− HmIgG vs. Ccr2−/− anti-IL-1α) measured by two-way ANOVA with Tukey’s multiple comparison test. d HE stained ear specimens collected from indicated mice on day 5 are shown. Bars indicate 500 μm. Data shown in (a–d) are representative of three independent experiments. Source data are provided as a Source Data file.

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