Fig. 7: Gγ subunit influences G protein responses in CGNs.
From: Specific pharmacological and Gi/o protein responses of some native GPCRs in neurons
a, b Percentage of change in BRET ratio between GαNluc and VenusGγ8 (a) or VenusGγ9 (b) induced by baclofen, brimonidine and CP 55940 in CGNs for the indicated Gi/o proteins. CGNs were co-transfected with the indicated VenusGγ and Nluc-tagged Gαi1, Gαi2, Gαi3, GαoA, GαoB or Gαz. Values are mean ± SEM from biologically independent experiments (baclofen and brimonidine, n = 5; CP 55940, n = 3) each performed in triplicate. c Scheme illustrating the difference in Gi/o (Gi1, Gi2, Gi3, GoA, GoB and Gαz) and indicated Gγ protein (Gγ2, Gγ8 and Gγ9) responses in CGNs for the receptors GABAB, CB1 and α2AR in presence of the indicated agonists. For each receptor and Gi/o sensor, the size of the circle is the percentage of change in BRET ratio upon the agonist stimulation, for GABAB (Figs. 2a and a, b), α2AR (Figs. 5e and a, b) and CB1 (Figs. 6b and a, b) normalized to the Gi/o sensor that has the highest response: for Gγ2, Gi1 for both the GABAB and CB1, and Gαz for α2AR; for Gγ8, GoA for GABAB and α2AR and Gi1 for CB1; and for Gγ9, Gi1 for GABAB and CB1, and Gαz for α2AR. For Gγ8, the empty circle indicates an increase in the BRET ratio in contrast to other conditions where a decrease BRET ratio is measured. The raw data and p-values are available in source data provided as a Source Data file.
