Fig. 3: GREB1 expression in the murine endometrium increases at the time of embryo implantation.

a Schematic representation of collection of uteri at different days of pregnancy (days post-coitum) in mice. b Representative images of GREB1 (green) localization in wild-type CD1 mouse uteri at the indicated days of pregnancy. Asterisk indicates the location of a blastocyst. G gland, LE luminal epithelium, S stroma, PDZ Primary decidual zone, SDZ Secondary Decidual zone; Scale bar:100 µm. White arrows, GREB1-positive cells; Red arrows, GREB1-negative cells; Blue dashed outline, decidual polygonal cells. c Representative images of GREB1 (green) localization at an implantation site and an inter-implantation site in a uterus from a wild-type CD1 mouse at 5 days. Middle schema (created with BioRender.com) depicts the implantation site and inter-implantation sites. In each time point, at least 5 independent samples (n = 5) from different mice were examined. All uteri were collected at 9:00 a.m. to 10:00 a.m. on the indicated day of pregnancy.