Fig. 3: Core cis-regulation circuit in parathyroids.

a Motif sequences from GCM2 peaks and matched known TF motifs. b Binding prediction enrichment in TSS-proximal regions (red) and TSS-distal regions (orange) for MAFB and GATA3. P-values by a one-sided Fisher’s exact test. c Predicted binding sites of GCM2 and MAFB at the distal enhancer (4.5 kb from TSS) of the PTH gene. GCM2 and MAFB are predicted to co-bind their core motif sites that are 40-bp apart. Y-axis: normalized read densities for GCM2 ChIP-seq (black), DNase-seq (blue), and H3K4me1 ChIP-seq (orange). The scale of the value is indicated in parentheses. d Schematic illustration of identifying key TF regulators working with GCM2. We analyzed TF footprints in open chromatin regions of the GCM2 gene (upper) and identified a set of TFs that co-regulate genes along with GCM2 (lower). e A core TF regulation circuit in parathyroids. TFs with black solid circles are auto-regulated. The red color intensity indicates the number of connections (in and out combined).