Fig. 2: Nanomotion signals vary depending on antibiotic concentration and strain variability. | Nature Communications

Fig. 2: Nanomotion signals vary depending on antibiotic concentration and strain variability.

From: Accurate and rapid antibiotic susceptibility testing using a machine learning-assisted nanomotion technology platform

Fig. 2

a Representative nanomotion recordings for E. coli ATCC-25922 (S, MIC = 0.0048–0.064 µg/ml) and E. coli BAA-2452 (R, MIC = 48–64 µg/ml) cells when treated with different CRO concentrations. A dose-response pattern is evident when comparing the slopes of the variance during the drug phase. b CRO concentration-dependent slopes during drug phase calculated by the formula log(x) = log(C) + at, where t is time, a is the slope of the common logarithm of the variance trend, and log(C) is the intercept. (1) and (2) represent the MICs of ATCC-25922 (0.047 µg/ml) and BAA-2452 (48 µg/ml), respectively, while (3) represents the control without CRO. Shown are mean and SD of triplicates per concentration. c Nanomotion signal variability for four susceptible and four resistant E. coli isolates (additional combinations can be found in Supplementary Fig. 5). Shown are representative recordings for each isolate. Data are available in the source data file.

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