Fig. 3: PARMS extend the α-helix promoting irreversible unfolding.
From: Alternative low-populated conformations prompt phase transitions in polyalanine repeat expansions
A Secondary chemical shifts for XS20 (red), XS23 (orange) and XS26 (green) at 25 °C. B 15N heteronuclear NOEs for XS20 (red), XS23 (orange) and XS26 (green) at 25 °C. Errors bars (<0.4) are not included for simplicity. The red shadows in the plots in A and B limit the α-helix in XS20. C CD spectra at 25 °C for XS20 (red), XS23 (orange) and XS26 (green) before and after heat denaturing at 95 °C (darker spectra in the plots). Protein concentration was 0.5 mM (A, B) and 0.017 mM (for XS23) and 0.036 mM (for XS20 and XS26) in C. Source data are provided as a Source Data file.
