Fig. 5: Pathogenic PARMs promote rapid phase transitions.
From: Alternative low-populated conformations prompt phase transitions in polyalanine repeat expansions
A Turbidity at 25 °C for 0.225 mM XS20 (red), XS23 (orange) and XS26 (green). Broken lines represent consecutive days of incubation. B Confocal fluorescent microscopy images showing co-localization of XS20 in XS26 condensates. XS26 was labeled with ATTO-488 (green) and XS20 with ATTO-565 (red). Scale bar: 5 μm. Data were biologically reproduced at least three times (see Supplementary Fig. 13). C Histogram distribution of the area (in μm2) of the condensates formed by the protein mixtures with respect to the time spent for the microscopic observations. Error bars (<0.3 in μm2) are not included for clarity. D TIRF-FRAP data for XS26 condensates (green), and for mixtures of XS26 + XS20 (brown) and XS23 + XS20 (orange) incubated for 6-18 minutes at 25 °C. FRAP data obtained at different incubation times was combined. Condensates showed marginal fluidity in all conditions tested and no fluorescence was recovered after bleaching. nXS26 = 64; nXS26+XS20 = 50; nXS23+XS20 = 16 (n: number of condensates). Data are presented as mean values +/−SD. Samples in B–D contained 0.1 mM XS23 and XS26 and 0.15-0.25 mM XS20. Source data are provided as a Source Data file.
