Table 1 List of oligonucleotides used for cloning
From: Alternative low-populated conformations prompt phase transitions in polyalanine repeat expansions
Forward | Reverse | |
|---|---|---|
XL20 insert | 5’ATTTCCAGGGATCCATGTATAAAATGGAATATTC3’ | 5’GTGGTGCTCGAGTTAGAACATACTGCTC3’ |
XL20 vector | 5´CTCGAGCACCACCACCACCACTG3´ | 5´GACATGGATCCCTGGAAATACAGGTTTTC3´ |
L20 insert | 5’ATTTCCAGGGATCCATCCGCACCACCTTCAC3’ | 5’GTGGTGCTCGAGTTAGAACATACTGCTC3’ |
S20 insert | 5’ATTTCCAGGGATCCGCTAAGTTTCGCAAGC3’ | 5’GTGGTGCTCGAGTTAGAACATACTGCTC3’ |
S20 vector | 5´CTCGAGCACCACCACCACCACTG3´ | 5’CTTAGCGGATCCCTGGAAATACAGGTTTTC3’ |
XS20 insert | 5’ATTTCCAGGGATCCGGCCCGGGGGGCCCGGG3’ | 5’GTGGTGCTCGAGTTAGAACATACTGCTC3’ |
XS20 vector | 5´CTCGAGCACCACCACCACCACTG3´ | 5’GGGCCGGATCCCTGGAAATACAGGTTTTC3’ |
XS23 | 5’GCAAGGGCGGTGCAGCAGCAGCGGCTGCGGCTG3’ | 5’CAGCCGCAGCCGCTGCTGCTGCACCGCCCTTGC3’ |
XS26 | 5’GCAAGGGCGGTGCTGCAGCAGCAGCAGCAGCG3’ | 5’CGCTGCTGCTGCTGCTGCAGCACCGCCCTTGC3’ |
C315 | 5’GAGCAGTATGTTCTGTTAACTCGAGCACCACC3’ | 5´GGTGGTGCTCGAGTTAACAGAACATACTGCTC’3’ |
