Fig. 3: Quantification of the reserve and recycling SV pool mobilities.

a, c Average mean square displacement (MSD; µm²) of the resting reserve pool (RP) of synaptic vesicles (SVs; black), RP SVs after stimulation (red) and the recycling pool of SVs (green) within the (a) presynapses and (c) axons. b, d Area under the MSD curve (AUC; µm²s) for (b) presynapses and (d) axons. e, f Average diffusion coefficient of the resting RP (black), RP SVs after stimulation (red) and the recycling pool of SVs (green) within the (e) presynapses and (f) axons. g, h Comparison of the density of detections in the axons and presynapses from (g) recycling and (h) reserve SVs labelled with anti-green fluorescent protein (GFP) Atto565 or 647N-tagged nanobodies (At565Nb or At647Nb) respectively, normalized by the area of the region of interest (ROI; traj/µm²). Data are displayed as mean ± SEM. Values were obtained from n  =  29 presynapses (Resting RP), n  =  33 presynapses (Stimulated RP) and n  =  28 presynapses (Recycling Pool) in (a, b), from n  =  19 axons (Resting RP), n  =  11 axons (Stimulated RP) and n  =  12 axons (Recycling Pool) in (c, d), from n  =  31 presynapses (Resting RP), n  =  30 presynapses (Stimulated RP) and n  =  26 presynapses (Recycling Pool) in (e), from n  =  18 axons (Resting RP), n  =  12 axons (Stimulated RP) and n  =  11 axons (Recycling Pool) in (f), from n  =  31 presynapses and n  =  45 axons in (g) and n  =  61 presynapses and n  =  74 axons in (h). Data were obtained from ≥3 independent neuronal cultures. Statistical comparisons were performed using the one-way ANOVA and Dunnett’s or Tukey’s multiple comparisons test in (b, d–f) and the unpaired two-tailed Mann–Whitney U test in (g) and (h). Source data are provided as a Source Data file.