Fig. 6: Synapsin TKO selectively affects reserve vesicle mobility.

a, b Area under the average mean square displacement (MSD) curve (AUC; µm²s) of presynaptic (a) recycling and (b) reserve synaptic vesicles (SVs) from wild-type (WT) and Synapsin triple knockout (SynTKO) hippocampal neurons. c, d AUC (µm²s) of axonal (c) recycling and (d) reserve SVs from WT and SynTKO hippocampal neurons. e, f Comparisons of the density of detections from WT or SynTKO neurons from (e) recycling and (f) reserve SVs, normalized by the area (traj/µm²). g, h Ratio of recycling to reserve SVs in (g) presynapses and (h) axons for WT (dark purple) and SynTKO (light purple) hippocampal neurons. Data are displayed as mean ± SEM. Values were obtained from n  =  28 WT presynapses and n = 16 SynTKO presynapses in (a); from n  =  29 WT presynapses and n = 36 SynTKO presynapses in (b); from n  =  12 WT axons and n = 5 SynTKO axons in (c); from n  =  19 WT axons and n = 18 SynTKO axons in (d); from n  =  32 WT presynapses and n = 14 SynTKO presynapses in (e); from n  =  65 WT presynapses and n = 38 SynTKO presynapses in (f). Ratios in (g, h) were obtained from the values in (e, f). Data were obtained from three biological replicates. Statistical comparisons were performed using the unpaired two-tailed Student’s t test in (a–d) and the unpaired two-tailed Mann–Whitney U test in (e) and (f). Source data are provided as a Source Data file.