Fig. 1: Cumulative mortality rate of irradiated Aedes mosquitoes exposed to three sex ratio over 8 days. | Nature Communications

Fig. 1: Cumulative mortality rate of irradiated Aedes mosquitoes exposed to three sex ratio over 8 days.

From: Mating harassment may boost the effectiveness of the sterile insect technique for Aedes mosquitoes

Fig. 1

The box plots present median values and quartiles, whiskers the 95% percentiles and dots the individual data points. One-tailed pairwise multiple comparisons were performed (P value adjustment with Tukey method) using the function emmeans () of the emmeans package to investigate the significance of the increase in cumulative mortality rate at different sex ratios as compared to the control. a Cumulative mortality rate of Ae. aegypti females increased with sex ratio and was 26.7% ± 14.0% at 8 days for a ratio of 99:1 as compared to 3.9% ± 2.4% in the control group (odds ratio = 0.141, SE = 0.021, z.ratio = −13.128, P < 10−4). No difference was observed in males (odds ratio = 1.313, SE = 0.227, z.ratio = 1.575, P = 0.256). Significant difference was also observed in females with sex ratio 49:1 (odds ratio = 0.544, SE = 0.100, z.ratio = −3.289, P = 0.002). The number of biologically independent replicates were n = 3 for the sex ratio 1:3 (control), n = 4 for ratio 49:1 and n = 6 for ratio 99:1; b, In Ae. albopictus, the tendency was even stronger. Significant difference was observed in females with sex ratio 49:1 (odds ratio = 0.106, SE = 0.049, z.ratio = −4.825, P < 10−4) and the cumulative mortality of females reached 40.0% ± 8.8% at 8 days for a ratio of 100:1 as compared to 3.8% in the control group (odds ratio = 0.106, SE = 0.049, z.ratio = −4.825, P < 10−4). Mortality of males also increased with the sex-ratio 50:1 (odds ratio = 0.093, SE = 0.039, z.ratio = −5.620, P < 10−4) and with the sex-ratio 100:1 (odds ratio = 0.081, SE = 0.034, z.ratio = −5.961, P < 10−4). The number of biologically independent replicates were n = 1 for the sex ratio 1:3 and n = 3 for ratio 50:1 and 100:1. Source data are provided in the Source Data file named “raw_data_lab&semi-field.xlsx”.

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