Fig. 1: Natural variation in BnaA9.NF-YA7 is associated with drought tolerance.
From: Natural variation in BnaA9.NF-YA7 contributes to drought tolerance in Brassica napus L
a Regional Manhattan plot of the BnaA9.NF-YA7 genomic region on chromosome A09. The 2-Mb genomic region on either side of the most significant SNP is shown. The lead SNP is shown with a red dot, and the other SNPs are colored according to their LD (r2 value) with the lead SNP. b Pairwise LD estimates for BnaA9.NF-YA7 gene locus. The green arrows denote the significantly associated SNP in BnaA9.NF-YA7. c The different varieties from five haplotypes survived after 14 days of drought treatment followed by 5 days of rewatering. Scale bars = 5 cm. d Comparison of survival rates among accessions of the five haplotypes. Values are means ± SD (Hap1: n = 27 accessions examined over ten independent experiments; Hap2: n = 188 accessions examined over ten independent experiments; Hap3: n = 14 accessions examined over ten independent experiments; Hap4: n = 9 accessions examined over ten independent experiments; Hap5: n = 11 accessions examined over ten independent experiments;). The lowercase letters indicate significant differences (P < 0.05, one-way ANOVA followed by two-tailed Fisher’s least significant difference (LSD) test). e Bean plot showing the water holding capacity of accessions from two alleles. P values were calculated with the two-tailed Student’s t-test (C: n = 21 accessions; G: n = 184 accessions). f The level of population differentiation (FST) across chromosome A09 between B. napus and B. rapa. g Expression levels of BnaA9.NF-YA7 in five haplotypes under 0.2 M d-mannitol treatment. The values are means ± SD (n = 3 biological replicates). Asterisks indicate statistically significant differences between Hap3 and Hap4 interaction based on two-tailed Student’s t-test (***P < 0.001 and P = 4.18 × 10−5, 3.50 × 10−4, 8.85 × 10−5, and 4.20 × 10−4 at the time points of 3, 6, 12, and 24 h). h Expression levels of BnaA9.NF-YA7 in Hap3 and Hap4 under 15% PEG treatment. The values are means ± SD (n = 3 biological replicates). Asterisks indicate statistically significant differences between Hap3 and Hap4 interaction based on two-tailed Student’s t-test (**P < 0.01, ***P < 0.001, and P = 1.78 × 10-5, 5.92 × 10−4, 8.71 × 10−5, 3.70 × 10−3, and 5.78 × 10−6 at the time points of 3, 6, 24, 48, and 72 h). n.s. not significant. Source data are provided as a Source Data file.
