Fig. 3: CCAAT-box variation affects the expression level of BnaA9.NF-YA7.
From: Natural variation in BnaA9.NF-YA7 contributes to drought tolerance in Brassica napus L
a GUS staining of ProBnaA9.NF-YA7Hap3:GUS and ProBnaA9.NF-YA7Hap4:GUS seedlings under normal conditions and 5 mM PEG, 0.5 µM ABA, and 0.2 M d-mannitol treatment. Scale bars, 2 mm, 5 mm or 500 µm. b, c GUS enzyme activity (b) and GUS expression level (c) between ProBnaA9.NF-YA7Hap3:GUS and ProBnaA9.NF-YA7Hap4:GUS seedlings under normal conditions. The values are means ± SD (n = 3 biological replicates). P values were calculated with the two-tailed Student’s t-test. d GUS enzyme activity of transgenic plants after fusion of the GUS reporter gene with BnaA9.NF-YA7 promoter fragments of different lengths. The values are means ± SDs (n = 3 biologically independent experiments). D1–D11 indicates BnaA9.NF-YA7 promoter fragments of different lengths. The lowercase letters indicate significant differences (P < 0.05, one-way ANOVA followed by a two-tailed LSD test). e SNP variation of the CCAAT-box within the promoter region of BnaA9.NF-YA7 between Hap4 and the other Haps. The sequences on the red lines are the target site. The red characters indicate substitutions. f Schematic diagram showing the constructs used in the transient transactivation analysis. g NF-Y trimers can activate the expression of BnaA9.NF-YA7, and the CCAAT-box variation affects the expression level of BnaA9.NF-YA7 between Hap3 and Hap4. The values are means ± SD (n = 3 biologically independent experiments). P values were calculated with the two-tailed Student’s t-test. Source data are provided as a Source Data file.
