Fig. 4: BnaABF3/4 s directly upregulates the transcription of BnaA9.NF-YA7.
From: Natural variation in BnaA9.NF-YA7 contributes to drought tolerance in Brassica napus L
a Y1H indicated that BnaABF3/4 s could bind the BnaA9.NF-YA7 promoter. b–d EMSA showed that BnaC7.ABF3 (c) and BnaA3.ABF4 (d) could bind the ABRE sites in the BnaA9.NF-YA7 promoter. The biotin-labeled probes are indicated in (b). e The fragments used in ChIP‒qPCR. f, g ChIP‒qPCR identified BnaC7.ABF3 (f) and BnaA3.ABF4 (g) as significantly enriched in the fragment containing the ABRE sites in the BnaA9.NF-YA7 promoter. Fold enrichments in (f) and (g) were calculated relative to input. The values are means ± SD (n = 3 biological replicates). h Schematic diagram showing the constructs used in the transient transactivation analysis. i BnaC7.ABF3 and BnaA3.ABF4 activates BnaA9.NF-YA7 independently or in the form of dimers. The values are means ± SDs (n = 6 biologically independent experiments). The lowercase letters indicate significant differences (P < 0.05, One-way ANOVA followed by two-tailed LSD test). Source data are provided as a Source Data file.
