Fig. 5: BnaA9.NF-YA7 directly targets BnaABF3s.

a Venn diagrams of the DEGs from transcriptomes and target genes of ChIP-Seq. b Genome browser view of normalized ChIP-Seq tags from the ChIP-Seq data and normalized RNA-Seq tags from the RNA-Seq data for BnaA9.NF-YA7, showing examples of BnaC7.ABF3 bound by BnaA9.NF-YA7 TFs. c, d GST pull-down (c) and co-immunicprecipitation assays (d) testing the interactions among BnaA9.NF-YA7 and BnaA9.NF-YB2/BnaA6.NF-YC4 in vitro and in vivo. e EMSA showing that NF-Y containing BnaA9.NF-YA7 could bind the CCAAT sites in the BnaABF3s promoter. Labeled probes containing the CCAAT element (Supplementary Fig. 18d, near TSS or within the coding region) were synthesized and labeled with biotin. The cold (unlabeled) probe and mutated CCAAT element (labeled) were used as a competitor and mutant probes, respectively. f Phenotype of B. napus seedlings grown in plastic bins (length × width × depth = 32 × 20 × 10 cm) under well-watered, drought stress, or rewatering conditions. WT indicates wild-type ZS 11 plants, DO indicates BnaA9.NF-YA7 and BnaC7.ABF3 double overexpression lines Bar = 2 cm. g The survival rate of BnaA9.NF-YA7-OE, BnaC7.ABF3-OE, and double overexpression lines were compared with that of the WT. The values are means ± SD (n = 50 plants examined over ten independent experiments). h Transpiration rate of BnaA9.NF-YA7-OE, BnaC7.ABF3-OE, and double overexpression lines were compared with WT under normal conditions. The values are means ± SD (n = 3 biological replicates). The lowercase letters indicate significant differences (P < 0.05, one-way ANOVA followed by a two-tailed LSD test). Source data are provided as a Source Data file.