Fig. 4: DRS analysis of CHIKV RNA does not reveal modification within the antibody-dependent m6A peak.
From: N6-methyladenosine modification is not a general trait of viral RNA genomes
a NanoConsensus scores along the CHIKV genome in HEK293T infected samples (12 h p.i, MOI of 4) obtained when compared to a CHIKV IVT sample control (using default parameters). In gray, non-significant positions; in blue, regions identified by NanoConsensus in only one replicate; in red, regions identified in both replicates. b NanoConsensus scores along the genomic (upper panels) and subgenomic transcript (lower panels) of CHIKV (using default parameters). In gray, non-significant positions; in blue, regions identified by NanoConsensus in only one replicate; in red, regions identified in both replicates. We should note that the potential modifications identified in one of the replicates at the very end of the transcript are typically false positives, caused by the high noise of nanopore signal at the ends of reads.
