Fig. 4: CLIC5 induces liposomal content mixing.
From: Chloride intracellular channel (CLIC) proteins function as fusogens
a Dose-response analysis of liposomal content mixing by CLIC5 using the carboxyfluorescein fluorescence unquenching assay. The time-dependent fluorescence increase reflects the dilution of carboxyfluorescein due to the fusion of loaded and unloaded liposomes. b DOC2B fails to induce liposomal content mixing in the carboxyfluorescein unquenching assay, regardless of the presence of calcium, consistent with its inability to form a fusion pore. c Liposomal content leakage analysis using the fluorescence monitoring of encapsulated NBD-glucose. No leakage from the liposome lumen following incubation with CLIC5 could be detected, indicating that the increase in carboxyfluorescein fluorescence (a) does not result from content leakage. For all experiments, data are presented as mean ± SEM, n = 3–4 independent experiments.
