Fig. 6: CLIC5 undergoes structural rearrangements upon acidification.
From: Chloride intracellular channel (CLIC) proteins function as fusogens
a Crystal structures of CLIC5-Δloop-WT (left) and CLIC5-Δloop-F34D (right). 2Fo-Fc electron density maps, contoured at 1σ, are provided for position 34, and residues within 5 Å are shown as sticks. b The difference in deuteration levels at the indicated time points between pH 5.5 and 7.5 for CLIC5-WT (upper panel) and CLIC5-F34D (lower panel). Due to the difference in the exchange rate at different pH values (exchange 100x slower at pH 5.5 than at 7.5), time points of equal exchange (20 s or 60 s at pH 7.5 and 2000s or 6000 s at pH 5.5, respectively) must be compared. c The HDX difference between the pH levels tested at the final time point is mapped onto the structure of CLIC5 (PDB 6Y2H). d The difference in deuteration levels at the indicated time points between CLIC5-WT and CLIC5-F34D at pH 7.5 (upper panel) and pH 5.5 (lower panel). (e) The HDX difference between CLIC5-WT and CLIC5-F34D at the final time point is mapped onto the structure of CLIC5 (PDB 6Y2H).
