Table 1 Crystallographic statistics
From: Chloride intracellular channel (CLIC) proteins function as fusogens
Data collection | ||
|---|---|---|
CLIC5-Δloop-WT | CLIC5-Δloop-F34D | |
Spacegroup | C2221 | C2221 |
Cell dimensions | ||
a,b,c (Å) | 130.13, 220.83, 45.02 | 129.69, 220.29, 45.48 |
α,β,γ (°) | 90, 90, 90 | 90, 90, 90 |
Beamline | ESRF ID30B | |
Wavelength (Å) | 0.886 | 0.886 |
Resolution (Å) | 2.10 | 2.51 |
Multiplicity | 16.7 (17.1) | 6.5 (6.8) |
Completeness (%) | 99.8 (99.5) | 99.6 (98.6) |
Mean I/σ(Ι) | 19.1 (1.4) | 11.8 (1.1) |
Rmeas (%) | 8.5 (184.3) | 12.6 (197.5) |
CC1/2 (%) | 100.0 (85.1) | 99.8 (50.3) |
Refinement statistics | ||
No. reflections (work/free) | 36476/2005 | 21694/1141 |
Resolution range | 42.56–2.10 | 42.42–2.51 |
Rwork/Rfree | 0.2321/0.2525 | 0.2438/0.2730 |
No. atoms | ||
Macromolecules | 3209 | 3205 |
Ligands | 10 | 5 |
Solvent | 49 | 25 |
average B-factor | ||
Macromolecules | 74.8 | 79.8 |
Ligands | 100.9 | 115.6 |
Solvent | 58.9 | 64.2 |
RMSD (bond lengths) | 0.005 | 0.005 |
RMSD (bond angles) | 0.71 | 0.74 |
Ramachandran outliers (%) | 0 | 0 |
