Fig. 6: Role in virulence of potential Lifestyle Associated Genes exclusively identified in Burkholderia plant pathogens.

a Phylogenetic analysis of the Burkholderia spp. (n = 17) associated with plant pathogenic lifestyle, based on concatenated alignment (2146 amino-acid positions) of 54 ubiquitously conserved proteins identified with PhyloPhlAn 3.0¹⁰² and visualized/annotated using iTOL¹⁰⁶. The phylogenetic tree is also coupled with species assignment and a sequence similarity (SS%) heatmap showing the distribution of the 13 selected LAGs. b Oryza sativa L. cv. Baldo seeds shoot length preinoculated with the wild-type strain (B. plantarii DSM 9509) and LAGs generated mutants (OD₆₀₀ = 0.5). Error bars correspond to the standard deviation of at least 14 different seeds from two independent experiments. The mean serves as the measure to define the center of the error bars. Statistically significant differences were determined by one-way ANOVA (P ≤ 0.05) followed by post hoc Tukey test and relative to the wild-type strain. c Disease symptoms displayed by rice seedlings 10 days after infection with B. plantarii DSM 9509 and LAGs mutants.