Fig. 3: Conformational dynamics of the Gea2 HDS arm and Sec7 domain.
From: Structural insight into an Arl1–ArfGEF complex involved in Golgi recruitment of a GRIP-domain golgin
a Superimposition of protomers A (in gray) and B (in pink) based on the rigid DCB and HUS region. The double-headed arrow indicates a 9° tilt between the two HDS arms. b The Sec7 domain is connected to the Gea2 main body by three structural elements that may facilitate motions: the HUS box in magenta, the N-linker in orange, and the C-linker in blue. c Superimposition of the HUS-Sec7 regions in Arl1–Gea2 (this study) (yellow and green) and Arf1–Gea2 complex (purple; PDB 7URO). The alignment is based on the stationary HUS domain. The Sec7 domain remains in place when bound to Arl1 but swings by 35° hinging around the HUS box during GEF catalysis in the Arf1-bound state.
