Fig. 6: Mutations in the binding surface of Arl1 for Gea2 affect cellular localization of Imh1.
From: Structural insight into an Arl1–ArfGEF complex involved in Golgi recruitment of a GRIP-domain golgin
a Localization of mCherry-Imh1 in arl1∆ cells expressing empty vector (pRS313), Arl1 WT or Arl1 mutants. b Localization of mNeonGreen (mNG) tagged WT Arl1 or Arl1 mutants. Scale bar is 2 μm. Quantification via Manders’ Coefficient of the proportion of mCherry-Imh1 (c) or mNG tagged Arl1 (e) colocalized with a late Golgi marker Sec7. For all quantifications, data from (n = 20) cells from three independent experiments of biological replicates were obtained and analyzed. Comparisons were calculated via a One-Way ANOVA followed by Tukey’s post hoc test. Error bars represent SD. d Illustration of Arl1 at TGN binding to Imh1 (PDB 1UPT) and to Gea2 using same surface region. The two structures are aligned by the lower left Arl1 and viewed from the membrane. Detailed interfaces are shown in Fig. 4a and Supplementary Fig. 14a. Source data are provided as a Source Data file.
