Fig. 2: Replication labelling with EdU and Click-iT chemistry in Spurr’s and Lowicryl embedded samples. | Nature Communications

Fig. 2: Replication labelling with EdU and Click-iT chemistry in Spurr’s and Lowicryl embedded samples.

From: In-section Click-iT detection and super-resolution CLEM analysis of nucleolar ultrastructure and replication in plants

Fig. 2

Plant root sections embedded in Lowicryl (A) and Spurr’s (C) resin labelled with Click-iT chemistry, with surface plots showing the relative background levels in the respective fixation and embedding conditions (B, D). Large field of view reconstruction of the SMLM image highlighting cells in different stages of DNA replication (E). Nuclei showing distinct replication patterns (F—early-S-phase, G—mid-S-phase and H—late-S-phase) are displayed in an overlay with the corresponding TEM micrographs. Spurr’s and Lowicryl embeddings were performed in two biological replicates (BR) and two technical replicates (per BR). Scale bar: A, C: 50 µm, E (+insets): 5 µm.

Back to article page