Fig. 7: Quantification of the FC and IRF diameter in wild-type and mutant A. thaliana.

Quantification of the size of IRFs in SMLM reconstruction (A), with structures highlighted after object detection (B). Quantification of FCs from TEM micrographs (D), with detected structures in (E). For IRF and FC quantification (C, F), n = structures analysed. Statistical significance was evaluated based on the non-parametric Kruskal–Wallis H-test (H-statistic = 12.3 for IRF analysis, p = 0.0021; H-statistic = 24.1, p = 5.83 × 10⁻⁶ for FC analysis; degrees of freedom = 2). Differences between groups were evaluated with the Wilcoxon rank-sum statistic (two-sided). The numbers correspond to the calculated area in nm². Experiments for wild-type, fas1 and nuc1 plants were performed in two biological and technical replicates. Experiments were performed in three biological replicates. Means are indicated by white circles, medians are indicated by the line inside the box (with minimum and maximum values are defined by the whiskers, percentiles (25% and 75%) are indicated by the top and bottom edges of the box. Confidence intervals (95%) for the median in the wild-type, fas1 and nuc1 IRF and FC measurements respectively are [90:142] for wild-type IRF data, [136:180] for fas1 IRF, [161:202.5] for nuc1 IRF, [250:313] for wild-type FC, [135:172.5] for fas1 FC, [157:229] for nuc1 FC: * sign indicates statistically significant difference between tested groups (p < 0.05). Source data are provided as a Source data file (source data file—sheet 1 for IRF quantification, sheet 2 for FC quantification). FC fibrillar centres, IRFs intranucleolar replication foci. Scale bar (A, B): 2 µm, (D, E): 1 µm.