Fig. 5: Evaluation of fibroblast cytotoxicity and inhibition of migration activity. | Nature Communications

Fig. 5: Evaluation of fibroblast cytotoxicity and inhibition of migration activity.

From: Endogenous stimuli-responsive separating microneedles to inhibit hypertrophic scar through remodeling the pathological microenvironment

Fig. 5

a Viability of NIH/3T3 fibroblasts treated with 5-Fu and 5-FuA at different concentrations for 48 h, respectively. b Viability of NIH/3T3 fibroblasts after incubation with 5-FuA, 5-FuA-Pep-MA and 5-FuA-Pro-Pro solutions for 48 h, respectively. c Quantification of wound healing rate in scratch assay. d Live/dead staining fluorescence images of NIH/3T3 fibroblasts after co-incubation with 5-FuA, 5-FuA-Pep-MA and 5-FuA-Pro-Pro solutions for 48 h, respectively. e Photographs of wound scratch assay at 24 and 48 h under different treatments. The scale bar in the last image can be applied to the others in the same panel. Representative images from n = 3 independent experiments. The data are presented as mean ± SD (n = 4 independent experiments in (a, b), n = 3 independent experiments in (c)). The data in a were analyzed by t-test, and the data in (b, c) were analyzed by one-way ANOVA. P-value: n.s. means no significance, *P < 0.05.

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