Fig. 7: The gene distribution and functional annotation analysis in full thickness rabbit ear HS model.

a Global gene expression distribution across 12 samples (4 groups, n = 3 biologically independent samples). The lower bound of box represents the first quartile (Q1; the 25th percentile) of the data, the line in the middle of the box represents the median (Q2; the 50th percentile) of the data, the higher side represents the third quartile (Q3; the 75th percentile) of the data, the lower whisker shows the minima and its variability, and the higher whisker shows maxima and its variability. b 2D PCA plot of the relationships among all samples. c 3D PCA plot indicating the separation of all samples into 4 clusters. d Barplot of DEGs between different groups. e Heatmap of DEGs among NS, HS, drug-loaded MNs and 5-Fu injection groups. f Volcano plot for DEGs between HS and NS groups. The green plots represent down-regulated genes, and red plots indicate up-regulated genes. g Volcano plot between drug-loaded MNs and HS groups. h Volcano plot between 5-Fu injection and HS groups. i Volcano plot between drug-loaded MNs and 5-Fu injection groups. j The significantly enriched BP of GO terms between HS and NS groups. k The significantly enriched CC of GO terms between HS and NS groups. l The significantly enriched MF of GO terms between HS and NS groups. m The most significantly enriched KEGG signaling pathways between HS and NS groups. The data in (j–m) were analyzed by hypergeometric distribution. The P-values were adjusted using the Benjamini-Hochberg method.