Fig. 3: StarPEG-(KA7)4 induces self-assembly of multiple DNA nanotubes into closed micron-scale DNA rings.
From: Triggered contraction of self-assembled micron-scale DNA nanotube rings
a Confocal overview image of self-assembled DNA nanotube rings formed from 50 nM DNA tiles (yellow, Atto633-labeled, λex = 640 nm) in presence of 500 nM starPEG-(KA7)4. Rings are highlighted with white arrows. Scale bar: 20 μm. b Representative confocal (top) and STED (bottom) images of an individual DNA ring formed from 50 nM DNA tiles (yellow, Atto633-labeled, λex = 640 nm) in presence of 500 nM starPEG-(KA7)4. Scale bar: 2 μm. c Representative confocal images of individual DNA rings formed at starPEG-(KA7)4 to DNA tile (yellow, Atto633-labeled, λex = 640 nm) ratios from 0.1 to 30. The DNA tile concentration is constant at 50 nM. Scale bar: 2 μm. d Histogram of the DNA ring diameters for starPEG-(KA7)4 to DNA nanotube ratios from 0.1 to 30 (n = (7, 15, 15, 15, 15) DNA nanotube rings per condition). Source data are provided as a Source Data file.
