Fig. 1: Labeling and morphological classification of ON bipolar cells.
From: Distributed feature representations of natural stimuli across parallel retinal pathways
a Diagram illustrating intravitreal injection (left), the adeno-associated virus (AAV) constructs carrying Grm6 promoter elements, Cre recombinase genes (middle), and the conditional allele of the Ai148 mouse strain (right). This allele features a TRE2 promoter, loxP-flanked STOP cassettes followed by GCaMP6f, and then a CAG promoter, lox2272-flanked STOP cassette, and tTA2. b The main dark-shaded branch depicts the number of mice and bipolar cells that met quality-control criteria during each data collection phase, while the lighter subsidiary branches detail the criteria for data rejection, with branch widths representing the number of rejections; the widths of the two blocks at left and center are proportional to the numbers of mice, right block is proportional to the number of cells. c Top, middle, and bottom images showcase en-face views of dendrites and axons, and side view of the inner plexiform layer (IPL), respectively. Co-stains include cone arrestin (CAR) in blue, labeling cone photoreceptor, and choline acetyltransferase (ChAT) in magenta, marking starburst amacrine cells. Scale bars (shared for axon and IPL images) are included. The swarm chart shows dendritic territories with (d) listing bipolar cell types and counts as BC5o (n = 7), BC5i (n = 7), BC5t (n = 5), XBC (n = 7), BC6 (n = 6), BC7 (n = 9), BC8/9 (n = 4) and axonal territories (e) as BC5o (n = 7), BC5i (n = 6), BC5t (n = 5), XBC (n = 8), BC6 (n = 7), BC7 (n = 9), BC8/9 (n = 4). BC5o and BC5i differentiation is based on the number of cones contacted18, shown in the inset. f IPL stratification profiles of all ON bipolar cell types, BC5o (n = 6), BC5i (n = 5), BC5t (n = 5), XBC (n = 6), BC6 (n = 6), BC7 (n = 8), BC8/9 (n = 3). The graph has seven color-coded traces, indicating the mean (±SEM). g This denotes the correlation coefficient of paired bipolar cell stratification profiles from connectomic reconstructions8. Source data for this figure are provided as a Source Data file.
