Fig. 1: The P2Y₁₄R of intestinal epithelial cell regulates human IBD and DSS-induced experimental colitis.

a P2ry14 expression in the colonic mucosa of healthy individuals or IBD patients from GEO database (using datasets GSE38713, GSE75214, GSE16879). b The immunofluorescent images of colon tissues from healthy control and UC or CD patients stained with P2Y₁₄R (red), EpCAM (green), and nuclear (DAPI, Blue), Scale bar = 100 μm (up), Scale bar = 20 μm (down). c RT-qPCR analysis of P2ry14 mRNA of intestinal epithelial cells in mice (n = 6 mice per group). d Western blot assay of P2Y₁₄R protein expression of intestinal epithelial cells in mice (n = 3 mice per group). e Representative colonic samples from control mice and DSS-treated mice were stained with P2Y₁₄R (red), EpCAM (green), and nuclear (DAPI, Blue), Scale bar = 50 μm (left), Scale bar = 20 μm (right). f Experimental flow chart. g Body weight change and DAI evaluation during the disease process (n = 6 mice per group). h The length of colons from P2Y₁₄R^fl/fl and P2Y₁₄R^△IEC mice after DSS treatment (n = 6 mice per group). i Analysis of serum FITC-dextran assay (n = 6 mice per group). j The spleen index of P2Y₁₄R^fl/fl and P2Y₁₄R^△IEC mice after DSS treatment (n = 6 mice per group). k The H&E staining in colon tissues of DSS-treated mice, Scale bar = 200 μm. The data represent the mean ± SD for a, c-d and h-j, the data represent the mean ± SEM for g. The p-values were determined by two-tailed Unpaired T test for a, c and d, two-tailed paired Student’s t -test for g, One-way analysis of variance (ANOVA) with Tukey multiple comparison test for h, i, j. For b, e, and k, each image was acquired independently three times, with similar results. Source data are provided as a Source Data file.