Fig. 2: Redox status modulates the expression and the activity of IDH2. | Nature Communications

Fig. 2: Redox status modulates the expression and the activity of IDH2.

From: Epigenetic modulators link mitochondrial redox homeostasis to cardiac function in a sex-dependent manner

Fig. 2

a Experimental scheme depicting the treatment of NRCMs with increasing concentrations of H2O2, and qPCR analysis of Idh2, Hmox1, Nqo1 and Osgin1 expression, normalized to untreated control. Equal amounts (5 ng) of total RNA were utilized for all qPCR reactions. Total RNA depicts the recovered amount of extracted RNA from the wells of H2O2-treated NRCMs, compared to untreated control. Horizontal dashed lines mark the mean levels in control samples. Values represent the average of 4 individual wells ±SEM. b Volcano plot for differentially succinylated peptides of mitochondrial metabolic enzymes in the LV of patients with ICM, obtained from publicly available mass spectroscopic data34. c Idh2 activity in cardiac mitochondria isolated from frozen LV myocardium of male Mlp-/- with or without the Idh2-activity-inhibitor, N-ethylmaleimide (NEM). d 2OG levels in the LV of male Mlp-/-. Bars represent mean ± SEM, analyzed with unpaired two-tailed t-test. The sample size is indicated on all figures. Source data are available in the Source Data file.

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