Fig. 2: DHCR7 inhibition suppresses lipid peroxidation. | Nature Communications

Fig. 2: DHCR7 inhibition suppresses lipid peroxidation.

From: Inhibition of 7-dehydrocholesterol reductase prevents hepatic ferroptosis under an active state of sterol synthesis

Fig. 2

Control (sgGFP) and DHCR7-ablated (sgDHCR7) Huh-7 cells were treated with RSL-3 (0.1 µM) for 6 h. The fluorescence intensity of C11-BODIPY581/591 was analyzed by flow cytometry. a Representative plot of C11-BODIPY581/591-stained cells. b The number of oxidized C11-BODIPY581/591-positive cells and the mean fluorescence intensity of oxidized C11-BODIPY581/591 were analyzed. (c, d) Control (sgGFP) and DHCR7-ablated (sgDHCR7) Huh-7 cells or A9944-treated cells were treated with RSL3 (0.1 µM) for 16 h, and lipid peroxides were measured by LC-MS/MS. a Data are representative and b means of three independent experiments and expressed as dot plots and means ± SEM. c, d Data are from four replicates. Statistical significance was calculated using two-way ANOVA with Tukey’s post hoc test.

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