Fig. 6: DHCR7 inhibition prevents ferroptosis-related liver diseases. | Nature Communications

Fig. 6: DHCR7 inhibition prevents ferroptosis-related liver diseases.

From: Inhibition of 7-dehydrocholesterol reductase prevents hepatic ferroptosis under an active state of sterol synthesis

Fig. 6

a DHCR7 expression in normal tissues and organs was analyzed using the RefEx database. b–e Liver samples and serum were obtained from hepatic ischemia-reperfusion or sham-operated C57BL/6J male mice treated with AY9944 (25 mg/kg) 1 h prior to open laparotomy. HE staining (b) and serum AST and ALT levels (c) were assessed. d Lipid peroxides in the liver and e 7-DHC in the liver and serum were assessed by LC-MS/MS. f, g C57BL/6J male mice were injected with 10 µg of px330 carrying a pair of Dhcr7-targeting gRNA or empty gRNA scaffold (NC) using a hydrodynamics-based procedure. PBS or acetaminophen (300 mg/kg) was intraperitoneally injected 7 days after gene transduction. Serum AST and ALT levels and HE staining were assessed. Data are collected from c 3–9 mice per group (n = 3 in vehicle/sham and AY9944/sham, n = 8 in vehicle/IRI, n = 9 in AY9944/IRI), d 6 mice per group, and e 3 mice per group. Statistical significance was calculated using two-way ANOVA with Tukey’s post hoc test. f–g Data are from 11 mice per group and statistical significance was calculated using two-tailed Mann-Whitney’s U test. Data are expressed as dot plots and/or means ± SEM.

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