Fig. 5: Deg1 inserts into the lateral tunnel of Doa10.
From: Substrate recognition mechanism of the endoplasmic reticulum-associated ubiquitin ligase Doa10
a Schematic diagram of Deg1-Ura and the predicted secondary structure of Deg1 using AlphaFold2. b In-vivo photocrosslinking experiment using Bpa-incorporated Deg1-Ura and wild-type Doa10. IP, immunoprecipitation. c As in (b), but with Bpa incorporated into a specific position in Doa10 instead of Deg1. d, e Structure of the lateral tunnel (d, front view; e, side view), highlighting positions tested for crosslinking in panel (c). Ribbons in yellow and green represent TMs 5–7 and L8/9c, respectively. Residues in magenta crosslink to Deg1-Ura3. f As in (c), but testing the effects of Trx1 fusion on crosslinking with 734Bpa of Doa10. g As in (c), but testing the effects of tunnel mutations on crosslinking with 734Bpa of Doa10. Data in (b, c, f, g) are representative of two independent experiments. Source data are provided as a Source Data file.
