Fig. 4: Host gene expression and child’s age.
From: Gene expression analyses reveal differences in children’s response to malaria according to their age
The volcano plots show the association between each human gene’s expression and the child’s age at the time of the infection, before (A) and after (B) adjusting for differences in immune cell composition using a quasi-likelihood negative binomial generalized model. Each dot represents one gene and is displayed according to its log10 p-value (y-axis) and fold-change (x-axis). Blue and red points represent differentially expressed genes that were more expressed in younger and older children, respectively, corrected for multiple testing using FDR (FDR = 0.1). Correlation of the proportion of neutrophils (C), B cells (D), NK cells (E), and Plasma cells (F) (y-axis), estimated from gene expression deconvolution, with the age of the child in years (x-axis) using linear regression (respectively, Pearson’s R2 = 0.06, p = 0.002; Pearson’s R2 = 0.13, p = 2.10 × 10−5; Pearson’s R2 = 0.13, p = 1.15 × 10−5; and Pearson’s R2 = 0.06, p = 0.004). Note that different ranges for the y-axis in (C, D, E, F) due to differences in cell proportions. *DEG = differentially expressed gene. (N = 136 individuals).
