Fig. 1: TaSG-D1^E286K regulates heat tolerance in Indian dwarf wheat.

a Phenotypic analysis of heat responses of HS2 and ND4332, NIL^TaSG-D1 and NIL^{TaSG-D1-E286K}, M2-1 and ND4332, Fielder and TaSG-D1^E286K-OE, as well as NC4 and IIA236. Bar, 1 cm. b Statistical analysis of plant survival rates in response to heat stress (42 °C for 3 days). Statistical analysis was performed with GraphPad Prism 7 (v7.00). Each bar represents standard deviation. ****P < 0.0001, ***P < 0.001 (n = 3 biologically independent experiments; unpaired two-tailed t test). c Phenotypic and statistical analysis of ND4332 and its mutant line M2-1 in response to heat stress at adult stage. Phenotype of representative plants are shown in upper panel, bar, 5 cm. Performance of harvested grains from representative plants (50 for each plant) are shown in middle panel. CK, normal conditions; HT, heat treatment; bar, 1 cm. Statistical analysis of 1000-grain weight was shown in lower panel, performing with GraphPad Prism 7 (v7.00). The box plots display the interquartile range, comprising the first quartile, median, and third quartile, while the whiskers extend from the minimum to the maximum values. P value represents the significant difference of yield loss in response to heat stress between two lines (n  =  18 biologically independent samples; unpaired two-tailed t test). Source data are provided as a Source Data file.