Fig. 4: OsUBC12’s role in regulating LTG mainly depends on its conservatived ubiquitination function.

a Formation of the ubiquitin thioester bond was analyzed for OsUBC12 and OsUBC12^C92A using anti-His and anti-Ub antibodies. b Time-course germination analysis of WT (KY131), OsUBC12-OE and OsUBC12^C92A-OE lines over 7 days at 15 °C. Values are means ± SE from three individual biological replicates (30 seeds per biological replicate). The data were statistically analyzed by two-tailed Student’s t-test (*P < 0.05, **P < 0.01). c Relative expression of OsUBC12 in seeds of OsUBC12-OE and OsUBC12^C92A-OE lines. The expression level of the control samples (WT, KY131) was set to 1. Values are means ± SE from three individual replicates (n = 3). The housekeeping gene OsUBQ5 was used as an internal control to normalize the data. The data were statistically analyzed using two-tailed Student’s t-test (*P < 0.05, **P < 0.01). Germination performance of WT (KY131), OsUBC12-OE and OsUBC12^C92A-OE seeds after 108 h on ½-MS agar medium containing 0 μM (d), 1 μM (e) or 2 μM (f) ABA. The germination inhibition rates of WT (KY131), OsUBC12-OE and OsUBC12^C92A-OE seeds under 1 μM (g) or 2 μM (h) ABA at three timepoints. Values are means ± SE from three individual biological replicates (20 seeds per biological replicate). The data were statistically analyzed using two-tailed Student’s t-test (*P < 0.05, **P < 0.01). Source data are provided as a Source Data file.