Fig. 5: AgRP neurons lacking miR-33 show increased mitochondrial density and elevated expression of miR-33 target genes.
From: microRNA-33 controls hunger signaling in hypothalamic AgRP neurons
a Representative images of electron microscopy (EM) imaging in sections of the arcuate nucleas from fed wildtype (WT) and miR-33AgRPiKO mice after high fat diet (HFD) feeding. b–e Quantification of mitochondrial size (b), aspect ratio (c), density (d), and coverage (e) from analysis of EM images of WT and miR-33AgRPiKO mice (n = 3 WT and 3 miR-33AgRPiKO). f Schematic representation demonstrating pulldown of ribosome associated mRNAs from AgRP neurons using RiboTag mice. Generated using BioRender.com. g Relative expression of actively translated miR-33 target genes in WT and miR-33AgRPiKO mice after overnight fasting (n = 5 WT and 4 miR-33AgRPiKO, hypothalamus from 4 mice were pooled per “n” sample in order to get enough material for RNA processing). All data represent mean +/- SEM. Statistical analysis assessed by unpaired 2-sided Student t-tests (b–g). Source data are provided as a Source Data file.
