Fig. 3: Sex-specific and pregnancy-specific cell type contributions to cfDNA. | Nature Communications

Fig. 3: Sex-specific and pregnancy-specific cell type contributions to cfDNA.

From: Cell type signatures in cell-free DNA fragmentation profiles reveal disease biology

Fig. 3

A Endothelial cell rank distribution grouped by tissue is plotted for healthy non-pregnant control samples. B Cell type rank log2 fold change (FC) between healthy males and healthy non-pregnant females is visualized for sex-specific tissues (i.e., prostate and uterus). Cell types were either significantly higher in males (green), significantly higher in females (purple) or not significant (gray). P-values were generated using a two-sided Wilcoxon rank-sum test and considered significant if the corrected Benjamini & Hochberg (BH) p-value ≤ 0.05. C Rank log2 FC between healthy non-pregnant and healthy pregnant individuals is plotted for each tissue. Cell types per tissue were either significantly higher in pregnant individuals (green), significantly higher in non-pregnant individuals (purple) or exhibited no significant difference (gray). D Rank correlations for healthy pregnant control sample “GC02” are shown for liver endothelial cells and extravillous cytotrophoblasts (EVTs) when down-sampled from 35-fold sequence coverage. E Trophoblast cell rank distribution grouped by sub-type is plotted for healthy pregnant control samples (n = 331). Boxplots indicate median, interquartile range (IQR), and whiskers for 1.5× IQR. F The mean EVT rank with a standard deviation error bar is shown across healthy pregnant controls (n = 331) sampled at different gestational ages (wks weeks), VCT villous cytotrophoblast, SCT syncytiotrophoblast, p proliferative, preg pregnant, NK natural killer, EC endothelial cell, β beta, α alpha. Source data are provided as a Source Data file.

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