Fig. 9: Synchrotron SAXS data reveal the stability of the bundled wide-spacing state depends on temperature. | Nature Communications

Fig. 9: Synchrotron SAXS data reveal the stability of the bundled wide-spacing state depends on temperature.

From: Complexes of tubulin oligomers and tau form a viscoelastic intervening network cross-bridging microtubules into bundles

Fig. 9

A SAXS data (open circles) and corresponding fits (solid lines) for a sample prepared with 2.0 mM MgCl2 added to the standard PIPES buffer at pH 6.8. The sample temperature was held at 37 °C for 30 min, quickly reduced to 21 °C and held for 30 min, then quickly cycled back up to 37 °C. Data was taken via 1-second x-ray exposures every 10 min. Fit lines are color-coded for the wide-spacing bundled (green) and the intermediate state (yellow). Raw data and corresponding fits are provided in the Source Data file.

Back to article page