Fig. 1: Rab4A activation promotes ANA, aPL, proteinuria, and GN in female B6.TC mice.
Effect of Rab4A activation and T-cell-specific inactivation were examined on antinuclear autoantibody (ANA; A), β2-glycoprotein I or apolipoprotein H (Apo-H; B), and anti-cardiolipin autoantibody (ACLA; C) production and proteinuria (D) in age-matched, 20- to 29-week-old, male and female mice homozygous for constitutively active Rab4AQ72L or lacking Rab4A in T cells Rab4AQ72L-KO, respectively. E Effect of Rab4A on the development of GN, GS, and % hyalinosis in female mouse sets at ~50 weeks of age. Scale bars are embedded into each representative microscopic image. Dot plots present individual mice. F Effect of Rab4A on the development of GN, GS, and % hyalinosis in male mouse sets at ~50 weeks of age. Kidneys were scored by an experienced renal pathologist blinded to mouse genotypes. Scale bars are embedded into each representative microscopic image. Dot plots present individual mice. 2-way ANOVA and Sidak’s post-hoc test p values are displayed for multiple comparisons of Rab4 WT, Rab4AQ72L, and Rab4AQ72L-KO mice within B6 control and B6.TC SLE strains. Overall 2-way ANOVA p values are shown in the header of each figure panel, while Sidak’s post-hoc test p values < 0.05 over brackets reflect comparison between experimental groups.
