Fig. 2: Patterns of gene expression in each cluster of Drosophila trachea. | Nature Communications

Fig. 2: Patterns of gene expression in each cluster of Drosophila trachea.

From: A single-cell atlas of Drosophila trachea reveals glycosylation-mediated Notch signaling in cell fate specification

Fig. 2

a–c, g–i Expression levels of marker genes in each cluster of tracheal cells. d,e, Confocal images of nls-GFP expression under the control of sal-Gal4 (d) or kni-Gal4 (e). f Fluorescent image showing cut-expressing spiracular branch and transverse connective. The pupal trachea was stained with an antibody against Cut. Arrows point to TC and SB. j Confocal image showing the expression of wg-Gal4-driven UAS-CD8:GFP in SB. k The expression of vein in VB is visualized by a reporter of vn-nlslacZ in which a nuclear lacZ is under the control of promoter of vein gene. Arrow indicates VB. i The expression of Bs-GFP in tracheal progenitors. Scar bars: 20 μm (d–f, j, l), 50 μm (k). Each experiment was repeated independently with similar results three times (d–f, j–l). DT dorsal trunk, DB dorsal branch, TC transverse connective, ASP air sac primordium, VB visceral branch, SB spiracular branch, PC progenitor cells.

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