Fig. 8: Intercellular communication in trachea cell populations. | Nature Communications

Fig. 8: Intercellular communication in trachea cell populations.

From: A single-cell atlas of Drosophila trachea reveals glycosylation-mediated Notch signaling in cell fate specification

Fig. 8

a, b Directional Notch signaling between different cell populations. Circle plot (a) and heatmap (b) showing Notch signaling among individual clusters. c, d FGF signaling in tracheal cells. Circle plot (c) and heatmap (d) depicting the transport of FGF in tracheal cells. e Cytoscape diagram depicting the activity of regulons in each cluster. f–i Expression of Su(H)-NRE-GFP reporter in DB (f, h) and PC (g, i) domain. NRE-GFP represents Notch activity under ND (f, g) or HSD (h, i) condition. Each experiment was repeated independently with similar results for three times. j Projection of scRNA-seq dataset from HSD group to ND group. k Dotplot representing expression profiles of signaling ligands in the presence or absence of HSD. DT dorsal trunk, DB dorsal branch, TC transverse connective, ASP air sac primordium, VB visceral branch, SB spiracular branch, PC progenitor cells, LT lateral trunk, GB ganglionic branches, ND normal diet, HSD high-sugar diet. Scar bars: 30 μm (f–i).

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