Fig. 2: Using pREF to measure errors in Illumina and ONT sequencing.
From: A universal molecular control for DNA, mRNA and protein expression
a Annotations of conu, gece and repe genes in pREF, with heat maps showing k-mer coverage, GC-content and repetitiveness. Error profile of (b) Illumina and (c) ONT alignments across the pREF sequence. d Histogram of individual k-mers ranked according to error rate for Illumina and ONT sequencing. e Individual error profiles for selected k-mers (ONT = Oxford Nanopore Technologies). a–e A single replicate from each sequencing method was used to create each plot. Source data are provided in a Source Data File.
