Fig. 3: Measuring the quantitative accuracy of synthetic conu genes.
From: A universal molecular control for DNA, mRNA and protein expression
a Schematic diagram illustrates the design and use of conu genes as quantitative controls. b Density histogram from k-mer counts for conu gene families illustrates the distribution of technical variation in 31-mer normalised read count, calculated using a sliding window approach. Bounds of technical variation are a visual representation of read count variation and are not based on a statistical calculation. c Quantitative accuracy of simulated, DNA and RNA sequencing using Illumina and ONT sequencing technologies as measured from conu genes (ONT = Oxford Nanopore Technologies). d Density plots show the spread of technical variation in conu read counts in DNA and RNA libraries, prepared for ONT and Illumina sequencing. Source data are provided in a Source Data File.
