Fig. 4: In vitro transcription of pREF mRNA controls.
From: A universal molecular control for DNA, mRNA and protein expression
a Synthetic control genes are preceded by a T7 promoter that enables in vitro transcription into matched mRNA controls. b Scatter plots indicate the fold-differences in k-mer sequencing error rates between RNA and DNA libraries for Illumina and ONT sequencing. c Violin plot illustrates the enrichment of sequencing errors at k-mers that form hairpins. d Use of synthetic conu gene during differential gene analysis of lung adenocarcinoma cells with torkinib. The synthetic RNA controls (coloured points) indicate the accuracy for detecting fold change differences in gene expression (grey) between treated and untreated cells. Source data are provided in a Source Data File.
