Fig. 5: Neuronal activity induces mitochondrial fission that is blocked by the Camkk2 inhibitor STO609. | Nature Communications

Fig. 5: Neuronal activity induces mitochondrial fission that is blocked by the Camkk2 inhibitor STO609.

From: Activity-dependent compartmentalization of dendritic mitochondria morphology through local regulation of fusion-fission balance in neurons in vivo

Fig. 5

a Mitochondrial fission and fusion was visualized in dendrites of primary cortical pyramidal neurons electroporated with a plasmid encoding matrix targeted photo-activatable GFP (mt-paGFP) and matrix targeted mScarlet (mt-mScarlet). At 14DIV timelapse imaging was performed following photoactivation of a small subset of dendritic mitochondria. The orange arrow points to a fusion event followed by a fission event (blue arrow). b Images of mt-paGFP following the indicated treatment at 0 and 15 minutes after photo-activation. Orange arrows point to fusion events, blue arrows to fission events. c Quantification of fission and fusion rates for dendritic photo-activated mitochondria in the indicated conditions demonstrating that activity increases the rate of mitochondrial fission through a Camkk2 dependent pathway. DMSO = 49 photoactivated dendritic ROIs from 11 neurons, 1.74 ± 0.24 fission and 1.74 ± 0.26 fusion events per 15 minutes (mean ± SEM). PTX = 49 photoactivated dendritic ROIs from 11 neurons, 3.45 ± 0.36 fission and 1.76 ± 0.24 fusion events per 15 minutes (mean ± SEM). PTX + STO609 = 52 photoactivated dendritic ROIs from 13 neurons, 1.12 ± 0.17 fission and 1.0 ± 0.17 fusion events per 15 minutes (mean ± SEM). p values are indicated in the figure following Kruskal-Wallis tests. Data are shown as individual points with mean ± SEM. Scale bar, 5 μm.

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