Fig. 5: Chemokine expression and crosstalk to mesenchymal and epithelial cells of DTA⁺ cells.

a Chemokine expression in epithelial lung cells from SRC mice before (day 0) and after injury (day 2). Genes with a mean expression of at least three counts per 10,000 in any cell type are colored. b Expression of chemokines upregulated in DTA⁺ cells compared to other epithelial cells after injury (MAST test, FC > 2 and p_adj < 0.05 in any DTA⁺ cell type). Asterisks denote p_adj value for significant DEGs (***p_adj < 0.001, *p_adj = 0.01). The percentage of cells expressing the gene are plotted against the average expression per cell type. c Ranking of significant signaling pathways based on differences of overall information flow (i.e. summed interaction strengths) within the inferred networks on day 0 and day 2. Left: relative information flow. Middle: absolute information flow (log scale). Right: zoom in on the absolute information flow of pathways with log(information flow) <0.1 at any time point. Signaling pathways are colored by the type of interaction (yellow: secreted signaling, purple: ECM-receptor, green: cell-cell contact). d–g Hierarchical plots showing the inferred communication network for EDN (d), IL6 (e), LIFR (f) and EGF (g) signaling. Circle sizes are proportional to the number of cells in each cell type, and line width corresponds to the interaction strength. The relative contributions of ligand-receptor pairs making up at least 1% of the pathway strength are shown on the right.