Fig. 5: NMRs display constitutive stabilisation of HIF-1α under normoxic conditions. | Nature Communications

Fig. 5: NMRs display constitutive stabilisation of HIF-1α under normoxic conditions.

From: Naked mole-rats have distinctive cardiometabolic and genetic adaptations to their underground low-oxygen lifestyles

Fig. 5

Quantification of HIF-1α protein expression using different antibodies against three different protein epitopes (Representative western blots in Supplementary Fig. 5). A Proteintech rabbit polyclonal anti HIF-1α antibody (Clone developed against protein sequence including amino acids 574–799 of the human HIF-1 alpha protein; GenBank: BC012527.2). B Abcam rabbit polyclonal anti-HIF-1α antibody (Clone developed against recombinant fragment. This information is proprietary to Abcam and/or its suppliers). C Novus Lab rabbit polyclonal anti-HIF-1α antibody (Clone developed against a fusion protein including amino acids 530–825 of the mouse HIF-1 alpha protein; Uniprot #Q61221). Experimental groups contain n = 4 animals and western blot analysis was performed using several technical replicates. HIf-1α protein expression normalised to tubulin. Statistical analysis was performed using unpaired t test (with Welch’s correction and two-tailed P values), and data are represented as mean ± SEM. Source data are provided as a Source Data file.

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