Fig. 5: Data output for a Flow-Set microsphere run.
From: Single drop cytometry onboard the International Space Station
a Raw data from five channels, collected in 10 μs intervals, visualized in the rHEALTH Viewer. The sample is Flow-Set microspheres. The top shows the full run, which lasts for 155 s prior to the appearance of the trailing edge air bubbles. The channel colors are as follows: purple (FSC), cyan (SSC), dark blue (blue channel), green (green channel), and orange (orange channel). The middle shows a zoom-in to 3 s of data, allowing individual events to be seen. The bottom is a 4 ms window showing a single event. The first peak is the transit of the 3 μm microsphere through the 405 nm laser (blue and green channels) and the second is through the 532 nm laser (FSC, SSC, and orange channels). b The software analyzes burst intensity for each peak after determining a suitable threshold (dashed lines) and baseline (bold dashed lines). For illustrative purposes, only the green and orange channels are shown. The burst intensity is integrated into the baseline. c The orange burst intensities are plotted on a histogram showing the number of counts (y-axis) at each log10 burst intensity bin (x-axis). A histogram gate (O1) highlights the singlet population. d XY scatterplot of the blue and green channels on a log10 versus log10 burst intensity plot. Individual populations are gated for statistics. BG1 outlines the single events and BG2 outlines double events. Source data filenames are provided in the Source Data File.
